Exiqon miRCURY LNA microRNA 原位杂交优化试剂盒(FFPE)
昨日，Exiqon推出了基于FFPE样本的原位杂交优化试剂盒，这将成为miRNA研究新手的不二选择。使用者可通过这一优化试剂盒来调整自己探针的最佳使用量和试剂配比，从而确保自身探针实验的成功。试剂盒中含有的基于5’和3’双地高辛标记的miRCURY LNA™ microRNA 探针具有高灵敏度和特异性，该试剂盒可适用于不同组织样本来源的microRNA检测. 此外，该试剂盒还配有专门用于FFPE样本的杂交等试剂，无需再为配制试剂而烦心。
试剂盒附有详细的说明书，并为确保实验成功提供了相应的建议。该验证优化试剂盒只需一天即可完成实验。
目前上市的八种原位杂交探针验证试剂盒，包括有阴阳性对照，杂交缓冲液和蛋白酶K，此外，在每个试剂盒中，还有一个具有组织特异性的miRCURY LNA™ microRNA原位杂交探针。
订购信息请联系吉泰公司订购。
图一：Principle of the miRCURY LNA™ microRNA ISH Optimization Kit (FFPE). First, the tissue is “opened” using Protease K. In the hybridization step, the DIG-labeled LNA™ probe binds specifically to its target microRNA. Alkaline phosphatase (AP)-conjugated anti-DIG antibodies are then added. This step is followed by NBT-BCIP development and optional counter-staining with Nuclear Red.
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Kit 1 |
Kit 2 |
Kit 3 |
Kit 4 |
Kit 5 |
Kit 6 |
Kit 7 |
Kit 8 |
| Brain |
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yes |
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| Eye |
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yes |
yes |
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| Muscle |
yes |
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yes |
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| Lung |
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yes |
yes |
yes |
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| Kidney |
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yes |
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| Liver |
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yes |
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yes |
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| Colon |
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yes |
yes |
yes |
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| Cervix |
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yes |
| Heart |
yes |
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yes |
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yes |
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| Mammary Gland |
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yes |
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yes |
| Lung cancer |
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yes |
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yes |
yes |
yes |
yes |
| Colorectal cancer |
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yes |
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yes |
yes |
yes |
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| Breast cancer |
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yes |
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yes |
yes |
yes |
yes |
| Kidney cancer |
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yes |
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yes |
yes |
yes |
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| Cervix cancer |
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yes |
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yes |
yes |
yes |
yes |
| Testis cancer |
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yes |
yes |
yes |
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| Cell entity |
myocyte |
varies |
hepatocyte |
neuron |
endothelial |
smooth
muscle |
smooth
muscle |
basal cells |
图二：Choosing the appropriate miRCURY LNA™ microRNA ISH Optimization Kit. The table indicates the tissue(s) in which each of the kits has been validated.
